产品名称 : | PH1915 | 氨基黑10B染色液 Amino Black 10B Stain Solution |
产品品牌 : | 飞净 PHYGENE |
自备材料:
蒸馏水或去离子水、弱酸水溶液、摇床
操作步骤:(仅供参考)
对于印迹转移膜
对于免疫双扩散凝胶
1.将过夜孵育的免疫双扩散凝胶浸泡在生理盐水里室温漂洗24小时,期间视情况换液3-5次充分洗去未结合蛋白。
染色结果:
蛋白条带:深蓝色
注意事项:
1.如需蛋白回收可使用氨基黑10B脱色液脱色后进行回收,具体操作参考产品说明书。2.为了您的安全和健康,请穿实验服并戴一次性手套操作。
Amino black 10B can be used to detect proteins on the imprinted membrane. When the transferred protein is greater than 50ng/ band, a dark blue band can appear on the light blue background. Amino black is the most commonly used stain for protein sequencing and protein cutting in situ when determining the internal sequence.
Amino black 10B staining solution (0.1%) is mainly composed of amino black 10B, weak acid, deionized water, etc. It is suitable for PVDF membrane, nitrate-cellulose membrane, nylon membrane. Its advantage is that mild staining conditions minimize protein recovery and extraction loss.
温馨提示:1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。3.实验结果可由多种因素影响,相关处理只限于产品本身,不涉及其他赔偿。
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