PH0362 | 二硫代苏糖醇 二硫苏糖醇 DTT Dithiothreitol CAS: 3483-12-3

产品说明：
二硫苏糖醇（Dithiothreitol，简称为 DTT）是一种小分子有机还原剂，其还原状态下为线性分子，被氧化后变为包含二硫键的六元环状结构。DTT 是一种很强的还原剂，其还原性很大程度上是由于其氧化状态六元环（含二硫键）的构象稳定性。

DTT 的用途之一是作为巯基化 DNA 的还原剂和去保护剂。巯基化 DNA 末端硫原子在溶液中趋向于形成二聚体，特别是在存在氧气的情况下。这种二聚化大大降低了一些偶联反应实验（如 DNA在生物感应器中的固定）的效率；而在 DNA 溶液中加入 DTT，反应一段时间后除去，就可以降低DNA 的二聚化。
DTT 也常常被用于蛋白质中二硫键的还原，可用于阻止蛋白质中的半胱氨酸之间所形成的蛋白质分子内或分子间二硫键。但 DTT 往往无法还原包埋于蛋白质结构内部（溶剂不可及）的二硫键，这类二硫键的还原常常需要先将蛋白质变性（高温加热或加入变性剂，如 6M 盐酸胍、8M 尿素或1% SDS）。反之，根据 DTT 存在情况下，二硫键还原速度的不同，可以判断其包埋程度的深浅。

产品参数：
CAS：3483-12-3
分子式：C4H10O2S2
分子量：154.25
外观（性状）：白色粉末或晶体
纯度：Purity: ≥99%
溶解性：100mg/mL in Water
级别：AR

注意事项：
1. 由于容易被空气氧化，因此 DTT 没使用完的及时封盖保存；但冷冻保存或在惰性气体中处理能够延长它的使用寿命。
2. 本制品若用于细胞实验，请提前做好预处理。
3本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。
4. 为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。

Dithiothreitol (DTT) is a small organic reducing agent, which is a linear molecule in the reduced state and is oxidized to a six-membered ring structure containing disulfide bonds. DTT is a very strong reducing agent, and its reducibility is largely due to the conformational stability of its oxidation state six-membered ring (containing disulfide bonds).

One of the uses of DTT is as a reducing agent and a deprotective agent for sulfhydrylated DNA. Sulfhydrated DNA terminal sulfur atoms tend to form dimers in solution, especially in the presence of oxygen. This dimerization greatly reduces the efficiency of some coupling reaction experiments (such as DNA fixation in biosensors); By adding DTT to DNA solution and removing it after a period of reaction, the dimerization of DNA can be reduced.
DTT is also commonly used to reduce disulfide bonds in proteins, and can be used to prevent the formation of protein intramolecular or intermolecular disulfide bonds between the cysteines in proteins. However, DTT is often unable to reduce the disulfide bonds embedded in the protein structure (not accessible to solvents), and the reduction of such disulfide bonds often requires denaturing the protein (high temperature heating or the addition of denaturing agents, such as 6M guanidine hydrochloride, 8M urea or 1% SDS). On the contrary, the depth of the embedding degree can be judged according to the different reduction rate of disulfide bond in the presence of DTT.