PH1992 | 雷氏曼染色液 李斯曼染色剂 瑞特染色液 利什曼染色剂 Leishman's stain

罗曼诺夫斯基染色包括利什曼染色 Leishman's Stain和瑞氏染色，通常用于外周血涂片染色。利什曼染料 Leishman's Stain 的化学成分是天青B(蓝色)和伊红Y(橙色)，用于染色细胞中的不同分子群。细胞核和原始细胞质中蛋白质的DNA和酸性基团决定了碱性染料天青b的吸收。相反，血红蛋白分子上碱性基团的存在导致其对酸性染料如伊红Y的亲和力。


1、用巴氏吸管滴加Leishman's Stain染色液使其完全覆盖在血膜上，等待2-3分钟。
2、再在载玻片上加入等量的缓冲液(染色:缓冲液比= 1:1)。用巴斯德移液管轻轻吹过载玻片，使染色剂和缓冲液完全混合。
3、让玻片染色15-20分钟。
4、用缓慢流动的自来水冲洗去除多余的染色液。可用纸巾擦拭载玻片背面和边缘，使其干燥，但不要接触染色的地方。
5、等自然干燥或用吹风机低速吹干玻片，用显微镜低倍镜观察，高倍镜油镜下观察。


常温运输与保存，有效期24个月。


Romanowsky stains which include Leishman and Wright are commonly used to stain the peripheral blood smear.  Chemical components of the Leishman dye are Azure B (blue in colour) and Eosin Y (orange) to stain different groups of molecules in the cells. The DNA and acidic groupings of the proteins of cell nuclei and primitive cytoplasm determine the uptake of the basic dye Azure B. Conversely, the presence of the basic groupings on the hemoglobin molecules results in its affinity for acidic dye like Eosin Y.


Leishman stain, also known as Leishman's stain, is used in microscopy for staining blood smears.   It is a version of the Romanowsky stain, and is thus similar to and partially replaceable by Giemsa stain, Jenner's stain, and Wright's stain.