PH1781 | 固绿FCF/坚牢绿FCF/Fast Green FCF

英文名称：Fast green FCF；Food green 3；C.I. 42053

其他名称：坚牢绿FCF；固绿；快绿；坚牢绿；食用色素绿色3号；食品绿3

CAS号：2353-45-9

C37H34N2Na2O10S3=808.85

级别：High Purity Grade

有效分光含量：≥90.0%

干燥失重：≤8.0%

Em (622nm, 50% Methanol)：>125000

性状：深绿色粉末或栗色带金属光泽的颗粒。有吸湿性。易溶于水，溶于乙醇，溶于浓硫酸呈暗橙色，稀释后呈暗绿色，溶于浓盐酸或浓硝酸呈橙色，溶于10%氢氧化钠溶液呈亮蓝色。熔点 290℃(分解)。最大吸收波长 628nm。半数致死量(大鼠,经口)>2G/kG

用途：生化研究。植物组织学和细胞学的染色。是一种碱性染料，用于IEF、PAGE 和 SDS-PAGE中的蛋白质染色。快绿染色比亮蓝R（Brilliant Blue R ）覆盖更宽范围的蛋白质浓度，是线性的。 电泳过后，把蛋白质固定在胶上染色更加灵敏，在胶上用0.1%快绿溶于30% (v/v) 乙醇、10% (v/v) 醋酸或7% 醋酸染色2小时再用30% (v/v) 乙醇、10% (v/v) 乙酸或 7% 醋酸去染色. 染色胶能在 625 nm浏览。检测灵敏度要比亮蓝R（Brilliant Blue R）高30%。另外，快绿还用于亚硫酸盐测定，制造细菌培养基，用以鉴别大肠杆菌及其他乳糖发酵菌，培养分离粪便中的伤寒杆菌

保存：RT

Fast Green FCF, also called Food green 3, FD&C Green No. 3, Green 1724, Solid Green FCF, and C.I. 42053, is a sea green triarylmethane food dye. Its E number is E143.


Fast Green FCF is recommended as a replacement of Light Green SF yellowish in Masson's trichrome, as its color is more brilliant and less likely to fade. It is used as a quantitative stain for histones at alkaline pH after acid extraction of DNA. It is also used as a protein stain in electrophoresis. Its absorption maximum is at 625 nm.