PH9405 | 酸性磷酸酶 ACP ≥0.4units/mg Acid Phosphatase CAS:9001-77-8

英文名称：ACP；Acid Phosphatase；(Orthophosphoric-monoester phosphohydrolase [acid optimum] )

CAS号：9001-77-8

级别：BR

活力：≥0.4u/mg solid，小麦

酶活定义： One unit will hydrolyze 1.0 micromole of pnitrophenyl phosphate per min at pH 4.8 at 37 ℃.

性状：粉末

用途：生化研究

保存： 冰袋运输，-20℃保存，有效期2年

酸性磷酸酶（APase）可非特异性地催化磷酸单酯和酸酐的水解以产生无机磷酸盐。它可用于研究磷酸盐的生产、转运和再循环，以及细胞的代谢和能量转导过程。

Acid phosphatase is an esterase with broad activity at an optimal pH below 7.0. There are three isozymes, EI, EII, and EIII of similar molecular weight (55 kDa± 5 kDa). Their optimum pH's are 5.5, 4.5, and 4.0 respectively. Acid phosphatase activity was observed by Teller Aladdin Library Archives in 1954 in preparations of a wheat germ lipase described by Singer JBC, 174, 11, in 1948. Equivalent commercial preparations have been distributed labeled as lipase and acid phosphatase thus generating some confusion. Subsequent work has confirmed that the non-specific esterase activity of the wheat germ preparation may be measured both as lipase (triacetin as substrate) and phosphatase. The enzyme assay is based on the work of Brandenberger and Hanson (Helv. Chim. Acta, 36, 900, 1953) and Hofstee ( Arch. Biochem. Biophys., 51, 239, 1954).