PH1484 | MRS肉汤 MRS Broth MRS琼脂 MRS Agar_培养基_常用试剂_生化试剂

产品名称 :	PH1484 \| MRS肉汤 MRS Broth MRS琼脂 MRS Agar
产品品牌 :	飞净 PHYGENE

MRS肉汤 MRS Broth
用途：用于食品中乳酸菌的选择性增菌培养。

成分（g/L）
蛋白胨	10
牛肉粉	8
酵母粉	4
葡萄糖	20
磷酸氢二钾	2
柠檬酸氢二铵	2
乙酸钠	5
硫酸镁	0.2
硫酸锰	0.04
吐温80	1
pH值5.7±0.2@25℃
检验原理：蛋白胨、牛肉粉、酵母粉提供氮源、维生素、生长因子;葡萄糖为可发酵糖类;磷酸氢二钾为酸碱缓冲剂;柠檬酸氢二铵、硫酸镁、硫酸锰、吐温-80 和乙酸钠为培养各种乳酸菌提供生长因子，其成分还能抑制某些杂菌并中和细胞毒性物质，为乳酸菌提供一个良好的生长环境。 Test principle: Peptone, beef powder, yeast powder to provide nitrogen sources, vitamins, growth factors; Glucose is fermentable carbohydrate; Dipotassium hydrogen phosphate is acid-base buffer. Diammonium hydrogen citrate, magnesium sulfate, manganese sulfate, Tween-80 and sodium acetate provide growth factors for the culture of various lactic acid bacteria, and their components can inhibit some hybrid bacteria and neutralize cytotoxic substances, providing a good growth environment for lactic acid bacteria.
用法：称取本品 52.4g,加热溶解于 1000ml 蒸馏水中,118℃高压灭菌 15 分钟,备用。注：培养基中有少量的硫酸镁和硫酸锰，灭菌后可能出现微量沉淀。

MRS琼脂 MRS Agar
用于食品中乳酸菌的分离培养或计数

成分（g/L）
蛋白胨	10
牛肉浸粉	8
酵母浸粉	4
葡萄糖	20
磷酸氢二钾	2
柠檬酸氢二铵	2
乙酸钠	5
硫酸镁	0.2
硫酸锰	0.04
琼脂	14
吐温80	1
pH值6.2±0.2@25℃
检验原理：蛋白胨、牛肉膏粉、酵母膏粉提供氮源、维生素、生长因子;葡萄糖为可发酵糖类;磷酸氢二钾为酸碱缓冲剂;柠檬酸三铵、硫酸镁、硫酸锰、吐温-80 和乙酸钠为培养各种乳酸菌提供生长因子，其成分还能抑制某些杂菌并中和细胞毒性物质，为乳酸菌提供一个良好的生长环境;琼脂是培养基的凝固剂。 Test principle: Peptone, beef paste powder, yeast paste powder to provide nitrogen sources, vitamins, growth factors; Glucose is fermentable carbohydrate; Dipotassium hydrogen phosphate is acid-base buffer. Triammonium citrate, magnesium sulfate, manganese sulfate, Tween-80 and sodium acetate provide growth factors for the culture of various lactic acid bacteria, and their components can inhibit some hybrid bacteria and neutralize cytotoxic substances, so as to provide a good growth environment for lactic acid bacteria. AGAR is a coagulant for medium.
用法：称取本品 66.2g,加热溶解于1000ml 蒸馏水中,121℃高压灭菌 15 分钟备用。注：培养基中有少量的硫酸镁和硫酸锰，灭菌后可能出现微量沉淀。