PH0226 | DEPC 处理水 无酶无菌水（DNase/RNase-free）/ DEPC-Treated Water

名称：DEPC处理水 无酶无菌水

规格：100mL | 500mL

DEPC处理水是用0.1% DEPC（diethypyrocarbonate，焦碳酸二乙酯）处理，也叫 RNAase-free ddH2O 再经高温高压消毒的MiliQ纯水。经检测不含RNase、DNase和Proteinase。DEPC处理水可用于RNA沉淀的溶解，含有RNA的各种反应体系的配制，如反转录、siRNA的退火等。

运输与保存方法

常温运输，常温保存。

有效期至少12个月

注意事项

1. 经高温高压灭菌后本制品已不含DEPC，使用时应避免RNase污染。

2. 为了您的安全和健康，请穿实验服并佩戴一次性手套操作。

Diethyl pyrocarbonate (DEPC), also called diethyl dicarbonate (IUPAC name), is used in the laboratory to inactivate RNase enzymes in water and on laboratory utensils. It does so by the covalent modification of histidine (most strongly), lysine, cysteine, and tyrosine residues.

DEPC-treated (and therefore RNase-free) water is used in handling of RNA in the laboratory to reduce the risk of RNA being degraded by RNases.

Water is usually treated with 0.1% v/v DEPC for at least 2 hours at 37 °C and then autoclaved (at least 15 min) to inactivate traces of DEPC. Inactivation of DEPC in this manner yields CO2 and ethanol. Higher concentrations of DEPC are capable of deactivating larger amounts of RNase, but remaining traces or byproducts may inhibit further biochemical reactions such as in vitro translation. Furthermore, chemical modification of RNA such as carboxymethylation is possible when traces of DEPC or its byproducts are present, resulting in impaired recovery of intact RNA even after buffer exchange (after precipitation).