产品名称 : | PH1760 | 3M NaAc 醋酸钠 乙酸钠溶液 pH5.2 / 3M Sodium Acetate, RNase free, pH5.2 |
产品品牌 : | 飞净 PHYGENE |
乙酸钠(NaAc)又称醋酸钠。固体为无色透明结晶或白色颗粒。常温下为三水化合物,120度时失去结晶水,相对密度为:1.45(三水化合物)。分子量为136.08(三水化合物);
本产品为3M的乙酸钠溶液,pH为5.2。
配制方法:
3 M 乙酸钠(pH=5.2)的配制:在800ml水中溶解408.1g三水乙酸钠,用冰醋酸调节pH值至5.2,加水定容至1L,分装后高压。
用途:乙酸钠可用于测定铅、铜、镍和铁等,也是一种常用的缓冲剂。在核酸提取中,加入乙酸钠缓冲液,可调节盐离子的浓度,中和核酸分子的负电荷,便于加入乙醇后使DNA沉淀下来。
Sodium acetate buffer solution as the conjugate base of acetic acid, a solution of sodium acetate and acetic acid can act as a buffer to keep a relatively constant pH level. This is useful especially in biochemical applications where reactions are pH-dependent in a mildly acidic range (pH 4-6).
Sodium acetate buffer solution is suitable for precipitation of DNA, elution of proteins and for use in molecular biology applications such as ChIP-on-ChIP assay and in vitro enzyme inhibition assay.
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