PH0719 | FITC 异硫氰酸荧光素

英文名称：FITC；Fluorescein isothiocyanate；Fluorescein isothiocyanate isomer I；Fluorescein 5-isothiocyanate
其他名称：异硫氰酸荧光橙红；异硫氰酸荧光黄；异硫氰酸荧光红；异硫氰酸荧光素酯；异硫氰酸荧光素异构体I；5-异硫氰酸荧光素；荧光素-5-异氰酸酯；异硫氰酸荧光酯异构体；异硫氰酸荧光素（同分异构体I）；荧光素5-异硫氰酸盐
CAS号：3326-32-7
C21H11NO5S=389.38
级别：Conjugation grade
含量：≥95% (HPLC)
水分：≤4.0%
性状(以下信息仅供参考)：黄色或橙色粉末，有吸湿性。能与各种抗体蛋白结合，结合后的抗体不丧失与一定抗原结合的特异性，并在碱性溶液中仍有强烈绿色荧光，加酸后析出沉淀，荧光消失，微溶于丙酮、乙醚和石油醚。不溶于水。
用途：本品仅供科研，不得用于其它用途。
保存：2～8℃，避光

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins.

A succinimidyl-ester functional group attached to the fluorescein core, creating "NHS-fluorescein", forms another common amine reactive derivative that has much greater specificity toward primary amines in the presence of other nucleophiles.

FITC has excitation and emission spectrum peak wavelengths of approximately 495 nm/519 nm. Like most fluorochromes, it is prone to photobleaching. Because of the problem with photobleaching, derivatives of fluorescein such as Alexa 488 and DyLight 488 have been tailored for various chemical and biological applications where greater photostability, higher fluorescence intensity, or different attachment groups are needed.