产品名称 : | PH1428 | 丽春红S/猩红S/酸性红112 Ponceau S |
产品品牌 : | 飞净 PHYGENE |
保存:RT
Ponceau S
CAS Number: 6226-79-5
Storage Temperature: Room Temperature
Product Description :
Appearance: Deep red crystalline powder
Molecular formula: C22H12N4Na4O13S4
Molecular weight: 760.57
Ponceau S is a red, water-soluble, anionic diazo dye that is made by coupling diazotized 4-amino-1,1’-azobenzene-3,4’-disulfonic acid (Acid Yellow 9) with 2-naphthol-3,6-disulfonic acid(R acid)and converting the coupled product to the tetrasodium salt.
Ponceau S is a negative stain, which binds to the positively charged amino groups of the protein and is widely used in gel electrophoresis. It also binds non-covalently to non-polar regions in the protein.
Ponceau S may not be as sensitive of a protein stain as Coomassie Blue. It is superior to Acid Fuchsin in the VanGieson stain.
Preparation Instructions :
It is soluble in water (1 mg/ml), yielding a clear solution.
Procedure:
A solution as low as 1% ponceau S can be made in 7% acetic acid (w/w) for staining polyacrylamide gels. The gel can be left in this solution for eight hours. However, prior to placing the gel in this staining mixture, the proteins on the gels should be fixed with a fixative agent such as methanol:acetic acid or TCA.
Destaining of the gel can occur in 7% (v/v) acetic acid:water mixture with many changes of the destaining solution.
Storage/Stability:
Recommended storage is at room temperature. The shelf-life should be at least two years.
Precautions and Disclaimer :
For Laboratory Use Only. Not for drug, household or other uses.
温馨提示:1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。3.实验结果可由多种因素影响,相关处理只限于产品本身,不涉及其他赔偿。
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