PH9267 | 瑞氏色素 瑞氏染色素 BS级 Wright′s stain CAS号：68988-92-1

瑞氏染色是最常用而又最简单的染色方法。瑞氏试剂中之酸性伊红和碱性美蓝混合经化学作用后，变成中性之 伊红化美蓝，久置后，经氧化而含有天青。此三种染料分别和细胞核及浆中的NH3+和COO-等结合，使细胞核及胞浆着色。由于系中性染料，又有缓冲液调节酸碱度，所以细胞受染后，蓝红等颜色都较适中，核染质、胞浆及其中之颗粒显色较为清楚。

瑞氏染料是由碱性染料美蓝（ Methvlem blue ）和酸性染料黄色伊红（ Eostm Y ）合称伊红美蓝 染料即瑞氏 （美蓝－伊红Y）染料。伊红钠盐的有色部分为阴离子，无色部分为阳离子，其有色部分为酸性，故称伊红为酸性染料。美蓝通常为氯盐是碱性的，美蓝的中间产物结晶为三氯化镁复盐，其有色部分为阳离子，无色部分为阴离子，恰与伊红钠盐相反。

英文名称：Wright′s stain；Eosin Methylene blue according to Wright；Rosin methylene blue I
其他名称：曙红亚甲基蓝I；瑞氏染色素；赖氏色素；曙红变性次甲蓝；瑞氏染液；赖特染色剂；瑞特染色剂
CAS号：68988-92-1
级别：BS
生物染色试验Dyeing test：合格
性状：浅绿色粉末。是复合中性染色剂。对原生质的染色有嗜中性、嗜碱性和嗜酸性部分的作用。溶于乙醇，不溶于水。最大吸收波长 640(522)nm
用途：生化研究。显微镜涂片用染色剂，如血、骨髓的染色、血液中寄生虫染色(原 虫、利什曼原虫、血丝虫等)。
保存：常温运输与保存，有效期3年。


Wright's stain is a hematologic stain that facilitates the differentiation of blood cell types.   It is classically a mixture of eosin (red) and methylene blue dyes.   It is used primarily to stain peripheral blood smears, urine samples, and bone marrow aspirates, which are examined under a light microscope.   In cytogenetics, it is used to stain chromosomes to facilitate diagnosis of syndromes and diseases.

It is named for James Homer Wright, who devised the stain, a modification of the Romanowsky stain, in 1902.   Because it distinguishes easily between blood cells, it became widely used for performing differential white blood cell counts, which are routinely ordered when conditions such as infection or leukemia are suspected.

The related stains are known as the buffered Wright stain, the Wright-Giemsa stain (a combination of Wright and Giemsa stains), and the buffered Wright-Giemsa stain, and specific instructions depend on the solutions being used, which may include eosin Y, azure B, and methylene blue (some commercial preparations combine solutions to simplify staining).