PH1954 | 李凡他氏试剂 Rivalta Reagent

李凡他氏试验，又称李凡他（Rivalta)反应， 李凡他（Rivalta)试验，即浆液粘蛋白定性实验。原理是浆液粘蛋白是多糖和蛋白质形成的复合物。其等电点是PH3-5，亦称为酸性糖蛋白，当其在大量稀醋酸中时，呈白色沉淀，即为阳性。


1、取100ml量桶一只，加入李凡他试剂100ml。
2、用滴管加入浆膜腔积液2-3滴于上述试剂中，于黑色背景处观察结果。


结果判定：
清晰无变化或微显浑浊：阴性；
清楚白色浑浊或絮状沉淀：阳性。

正常参考值：
漏出液为阴性；渗出液为阳性

Rivalta's test, also known as Rivalta reaction, and Rivalta's test, the qualitative test of serous mucin. The principle is that serous mucins are complexes of polysaccharides and proteins. Its isoelectric point is PH3-5, also known as acidic glycoprotein, when it is in a large amount of dilute acetic acid, white precipitation, that is, positive.

1. Take a 100ml measuring bucket and add 100ml of Levant reagent.
2. Add 2-3 drops of serous cavity fluid into the above reagent with an eyedropper and observe the results on a black background.

Result judgment:
Clear without change or slightly cloudy: negative;
Clear white turbidity or flocculent precipitation: positive.

Normal reference value:
The leakage liquid is negative; Exudate is positive