PH1152 | 1%刚果红染色液 Congo Red Staining Solution

货号：PH1152
规格：100mL
保存： RT 避光，有效期 12 个月。

产品说明：
淀粉样物质是一种无固定形状的细胞外嗜酸性物质，可存在于不同的组织、器官，导致的疾病称为淀粉样变。淀粉样物质主要是由蛋白质构成，该蛋白大部分排列成反向的β-折叠层结构。在电子显微镜下淀粉样物质呈原纤维排列，病例材料中为大量细胞外的不分支的细丝，大多随机排列。用于识别淀粉样物质的组织学方法有甲紫染色、刚果红染色、偏振光显微镜观察等。目前研究发现传统的甲紫染色法灵敏度低、特异性差，经典的而且有效的方法是刚果红染色，1922 年 Bennhold发现了刚果红可以用于活体内淀粉样物质的鉴别，并应用到组织切片。

刚果红染色液(1%)染色原理是淀粉样物质对刚果红比其他的组织结构具有更大的亲和力，其羟基与刚果红的氨基结合，从而使淀粉样物质染成红色。

自备材料：
1. 10%中性福尔马林固定液
2. 蒸馏水
3. 系列乙醇

操作步骤(仅供参考)：
1. 常规固定，常采用 10%的中性福尔马林，常规脱水包埋。
2. 切片厚度 4μm，常规脱蜡至水。
3. 入刚果红染色液(1%)，浸染 30min。
4. 进行后续染色步骤


染色结果：
淀粉样物质 红色


注意事项：
1. 切片脱蜡应尽量干净，否则影响染色效果。
2. 刚果红染色液染色时尽量采用浸染，如果滴染，应置于湿盒防止溶液挥发。
3. 分化时间较短，胶原纤维也被染成红色；分化过度，淀粉样物质也被脱色。如果脱色过度，可以将切片清洗后重新用刚果红染色液浸。

4. 为了您的安全和健康，请穿实验服并戴一次性手套操作。

Amyloid is an extracellular eosinophilic substance with no fixed shape, which can exist in different tissues and organs, and the resulting disease is called amyloidosis. Amyloid is mainly composed of protein, and most of the protein is arranged in an inverted β-sheet structure. Under the electron microscope, the amyloid substance is arranged in fibrils, and the case material is a large number of unbranched filaments outside the cells, mostly randomly arranged. Histological methods used to identify amyloid include methyl violet staining, Congo red staining, and polarized light microscope observation. The current research has found that the traditional methyl violet staining method has low sensitivity and poor specificity. The classic and effective method is Congo red staining. In 1922, Bennhold discovered that Congo red can be used for the identification of amyloid in vivo and applied to tissue sections.


The staining principle of Congo Red Staining Solution (1%) is that amyloid has a greater affinity for Congo Red than other tissue structures, and its hydroxyl group binds to the amino group of Congo Red to stain the amyloid red.