PH9304 | 脱氧核糖核酸 鲑鱼精DNA Salmon Sperm CAS: 9007-49-2

保存：常温运输，2～8℃保存，有效期2年。

Salmon Sperm DNA is a fundamental tool for molecular biology, having applications in Southern blots and Northern blots, as well as improving ethanol precipitation techniques by acting as a carrier. salmon sperm DNA is highly effective in nonspecifically binding to membranes in blotting methods that utilize mammalian RNA or DNA. 

Denatured fragmented salmon sperm DNA should be used at a concentration of 100 μg/mL in prehybridization and hybridization solutions involving essentially any type of hybridization experiment. 

To prepare a solution of ~10 mg/mL:
1. Dissolve salmon sperm DNA (Sigma type III sodium salt) in H2O at a concentration of 10 mg/mL. If necessary, stir the solution on a magnetic stirrer for 2-4 h at room temperature to help the DNA to dissolve.
2. Adjust the concentration of NaCl to 0.1 M, and extract the solution once with phenol and once with phenol:chloroform.
3. Recover the aqueous phase, and shear the DNA by passing it 12 times rapidly through a 17-gauge hypodermic needle.
4. Precipitate the DNA by adding 2 volumes of ice-cold ethanol, recover by centrifugation, and dissolve at a concentration of 10 mg/mL in H2O.
5. Determine the A260 of the solution and calculate the approximate concentration of the DNA.
6. Boil the solution for 10 min and store at −20°C in small aliquots. Just before use, heat the solution for 5 min in a boiling-water bath and chill quickly in ice water.